Directional cDNA library construction assisted by the in vitro recombination reaction
نویسندگان
چکیده
منابع مشابه
Directional cDNA library construction assisted by the in vitro recombination reaction.
We report here a new directional cDNA library construction method using an in vitro site-specific recombination reaction, based on the integrase-excisionase system of bacteriophage lambda. Preliminary experiments revealed that in vitro recombinational cloning (RC) provided important advantages over conventional ligation-assisted cloning: it eliminated restriction digestion for directional cloni...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2001
ISSN: 1362-4962
DOI: 10.1093/nar/29.4.e22